Parasitologia Clinica De Craig Faust 3 Ed Rev __LINK__

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Parasitologia Clinica De Craig Faust 3 Ed Rev

cfs/me is a debilitating, complex, multisystem disorder which presents with chronic fatigue, cognitive dysfunction, and immune dysregulation. to date, the aetiology of cfs/me remains unknown and no clinical tests have been developed to diagnose cfs/me. extracellular vesicles (evs) are small membrane-derived particles (50-200nm) released by cells into the extracellular environment. they carry cell-derived information which is transferred between cells in order to modulate their functions. evs are present in various body fluids and have been shown to be a valuable source of biomarkers. however, there are few studies describing the nature of evs in patients with cfs/me. we hypothesize that blood-derived evs in patients with cfs/me might be biomarkers of disease activity and/or progression. we will collect blood samples from cfs/me patients and healthy volunteers to characterize circulating evs. plasma samples were obtained from patients with cfs/me and healthy volunteers. evs from plasma samples were isolated by ultracentrifugation. the presence of evs was confirmed by transmission electron microscopy. nanoparticle tracking analysis (nta) was used to measure evs size and concentration. the ev-derived proteins, mrna and mirna were analyzed by western blotting, qrt-pcr and ngs, respectively. the relationship between plasma ev levels and clinical data was investigated using statistical methods. results will provide new insights into the pathophysiology of cfs/me and will be used to plan future clinical trials and non-invasive diagnostics of cfs/me.

discussion: most of the studies on evs focus on their biological functions and the mechanisms involved in their pathogenic role in different diseases. however, there are few studies that demonstrate the use of circulating evs as a valuable biomarker in clinical practice. the present study shows a higher concentration of evs in the plasma of cfs/me patients compared to healthy volunteers. the characterization of circulating evs by nta allowed us to detect a bimodal distribution of circulating evs in both groups.

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