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The development and use of practical tools for the expression of proteins in bacteria has been limited by a lack of reliable ways to suppress the expression of unwanted genes. In mammalian cells, the introduction of antisense constructs can be used to achieve suppression, but in bacteria this has been limited to suppress the production of toxins and enzymes that either act as effectors or affect essential cellular processes. Here, we describe the use of plasmid-based and gene-based systems for the suppression of gene expression in gram-negative bacteria.Hornet sting is no joke
Its irritating to be stung by a hornet.
But you know what’s worse? being kicked in the face by one of those hornets right when you’re sleeping, or being stung by a nest of them.
One man lost a rather expensive toy after being stung by a hornet’s nest, and we can only imagine the pain he must have been in.
The unlucky toy owner was visiting his parents at the time, and had just fallen asleep on a sofa when one of the nests was shaken loose.
The family have not revealed the kind of toy it was, only that it was expensive, and we can only assume it’s a piece of popular culture, like say a smartphone.
We honestly can’t think of anything more painful than being kicked in the face by a hornet, and while we did find a video of a dog being stung by the same thing, and watching it made us think of the poor dog owner and wish we were joking.
If your got one of these, why not kick it further
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