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Bioengineering 2012
August 16, 2012. My name is Charles Edward. I’m a first year biology student at the University of Oregon and my research, carried out during a summer internship with Joshua Winicki, has been published in the journal General and Comparative Endocrinology.
In a study published earlier this year, Joshua Winicki and I showed that the grasshopper Chorthippus japonicus expresses two sets of insulin-like growth factors, one somatogenic and the other autogenic. The researchers also found that they could manipulate the growth and reproduction of the animals through application of the somatogenic hormone. In the following studies, we sought to identify the regulatory signals that indicate when and where the somatogenic signal is active.
For these studies, we used electroporation, in which DNA is delivered to individual cells through brief electroporation. DNA was delivered to the somatogenic cells of grasshopper embryos using an existing transgenic line which carries the green fluorescent protein as a marker. We observed that the markers visible in somatogenic cells in the grasshopper embryo were labile. Thus, we designed experiments to measure the half-life of the protein markers, in order to attempt to quantify the rate at which DNA from the somatogenic cells is lost, and to track the movement of such DNA into and out of somatogenic cells.
We were able to establish a half-life of 16 minutes for the red fluorescent protein marker in vivo, but could not measure the half-life of the green fluorescent protein because it could only be used for morphological studies. In vitro experiments, using the marker in the form of plasmid DNA rather than RNA, suggested that the protein was degraded at a rate of 22 minutes. We predicted that if the somatogenic cells were nonautoregulatory and had no need for their own growth factor, the marker would be continuously produced and degraded, and would be present at a constant low level. Our electroporation studies showed that the DNA was degraded at a slightly lower rate than predicted, suggesting that there is some posttranscriptional control on the degradation of these proteins.
We
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Tertullian (150-220) studied at Carthage and Rome, and took no interest in anything in the modern sense of the term. He was a firm believer in the Epicurean doctrine of the harmony of the soul with the body, which was very popular among the Romans and had many followers in the early church. His works, though, are the foundations of early Latin Christian theological and ethics. With Epictetus he established the position that Christianity was a complete system of morality. His writings are clear and concise and are a good introduction to the life and thought of ancient Christianity.
Olivier de Serres (c 1520-1590), the royal mathematician, paid dearly for his dualism in anti-papist sentiments. The bishop of Garnes, Gorges, induced the king to condemn Serres to prison for his heterodoxy, where he died of hunger. The archbishop of Narbonne, however, released him from prison, and the grand master of the Teuton Order instituted a memorial service in his honor. This act earned the archbishop the enmity of the hierarchy, for the Teuton masters were in league with the Huguenot party. The archbishop was imprisoned, tortured and finally killed.
The philological school [ le phênèphragistique ] concentrated on the grammar of the Latin language, as a basis for the study of the traditional Latin authors of the Catholic Church. In this school, the grammar of each ancient writer was the first subject. The philological school was more than a series of linguistic discussions, but was a training for the priestly and missionary priesthood. The chief representatives of the earliest times of this school, in the first part of the 17th century, were Raimondo Mattei of San Giorgio in Velabro (1562-1632), and Odoardo Filarete (1586-1669), bishop of Tivoli. At first, little was accomplished beyond the restoration of Greek translations of ancient authors, but Mattei succeeded, through the chief defence of the deity of Christ, in showing its unity with the creeds of the Catholic Church and thus in winning many new converts. The integrity of the classical authors, which in the hands of the Renaissance philologists had often been confused and distorted, was defended by Filarete, a priest who had studied in Rome and had been appointed professor of Latin literature at the University of Leyden. His own works illustrate his
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