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The esterase gene (EST2) of Streptomyces sp. ZJ07-6, which encodes the positive and weak acid esterase II of this strain, was cloned by screening a genomic library with an oligonucleotide probe with DNA sequence homology to the active site of Est2. The nucleotide sequence of Est2 was derived from the DNA sequence of a subclone (Est2c) determined by sequencing of genomic clones and EST2c contains an open reading frame of 1,120 bp encoding a protein of 373 amino acids with a calculated molecular mass of 41.9 kDa. A peptide sequence homology search revealed that the amino acid sequence of Est2 is homologous to other amino acid sequences of type I alpha-sulfatase family esterases. The EST2 gene was expressed in Escherichia coli and the expressed protein was purified and shown to be active. Homology modeling of the deduced tertiary structure of the active site region of Est2 showed that it is composed of five loops, the N-terminal beta/alpha barrel, the C-terminal alpha/beta barrel, the proline bridge and the catalytic center loops. The modeled structure of Est2 is significantly similar to those of other type I alpha-sulfatases and has a negative charge in the catalytic center loop of Est2. Based on these results, it was concluded that Est2 is a member of the esterase family and the active site contains five loops named A-F loops. UNPUBLISHED
UNITED STATES COURT OF APPEALS
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Problems when trying to run “git checkout” after cloning a repository
I am having trouble with git checkout. I cloned a repository and then tried to use git checkout to specify the branch I wish to work on. Unfortunately, the git checkout command always returns with an error. The error I’m receiving is:
error: pathspec’sources/C/sources’ did not match any file(s) known to git.
A:
git checkout only works on branches, not for checking out single commits. It’s used to specify a specific branch from which to check out.
See also the branch command.
1. Field of the Invention
The present invention generally relates to an electrophotographic image forming apparatus and a fixing device installed in the electrophotographic image forming apparatus. More specifically, the present invention relates to a fixing device having a layered-type belt including an electroconductive layer.
2. Description of the Related Art
As a fixing device employed in an electrophotographic image forming apparatus, a fixing device having a layered-type belt (hereinafter referred to as a “belt fixing device”) capable of reducing the possibility of occurrence of an offset image caused by toner scattering can be employed. In such a belt fixing device, a rubber elastic layer is provided on a circumferential surface of a support body made of a metal or the like, and the rubber elastic layer has a belt shape. Furthermore, a heat source such as a halogen heater is embedded in the support body, and the support body is supported by a rotatable support shaft. Moreover, the belt fixing device is wound around the support shaft along a predetermined circumferential direction
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