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This study complements earlier work by the same group which first described how the microtubule network oscillates. This oscillation may coordinate the events of mitosis and cytokinesis, and has been found in all eukaryotic cell types studied thus far.
In this study, the authors measure the forces of the microtubule network in single cells. For this purpose, they integrated an optical trap with a fluorescence microscope, and then imaged the physical forces exerted by the microtubules as a cell undergoes mitosis.
They observe that within a set interval of time during mitosis, the microtubule network generates a force sufficient to cause individual chromosomes to depart the centrosome (the organizing microtubule center of the cell). They then go on to show that these forces will exert a component of force, or torque, on the cell membrane that is sufficient to pull one end of the cell away from the other. This force, they say, is the only force that can cause a cell to divide at the particular location of its membrane (mid-plane of the cell). The authors’ observations explain how the directional patterning that occurs during cell division and their finding that the mid-plane division is the default in all cells is consistent with the finding that the microtubules are dynamized during mitosis.
The group’s results are consistent with earlier measurements and experiments in *Xenopus* eggs and egg extracts. Many single cells have now been measured with the same camera and optical trap to measure
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